■
A Plant Orthologue of RNase L Inhibitor (RLI) Is Induced in Plants Showing RNA Interference
Authors: Braz, Antonio1; Finnegan, Jean2; Waterhouse, Peter2; Margis, Rogério3
Source: Journal of Molecular Evolution, Volume 59, Number 1, July 2004 , pp. 20-30(11)
Publisher: Springer
* < previous article
* |
* next article >
* |
* View Table of Contents
Key:
Free Content - Free content
New Content - New Content
Subscribed Content - Subscribed Content
Free Trial Content - Free Trial Content
Abstract:
RNase L inhibitors (RLIs) correspond to a group of soluble proteins from the large ATP binding cassette (ABC) family of proteins. Structurally, RLIs have an N-terminal Fe–S domain and two nucleotide binding domains. Orthologous RLI sequences with more than 48% identity have been found from Archea to Eukaryota, but have not as yet been identified in Eubacteria. Some organisms, like Arabidopsis thaliana and human, have paralogous genes with differential expression patterns, the function of which remains to be determined. Expression of Arabidopsis RLI2 was slightly increased in transgenic plants showing RNA interference, suggesting a role in this pathway
Keywords: RNase L inhibitor; RNAi; PTGS; RNA silencing
Document Type: Research article
DOI: 10.1007/s00239-004-2600-4
Affiliations: 1: LGMV―Laboratório de Genética Molecular Vegetal, Departamento de Genética, Instituto de Biologia, CCS, Universidade Federal do Rio Janeiro, 21944–970, Ilha do Fundão, Rio de Janeiro, Brasil, 2: Plant Industry, CSIRO, Canberra, ACT, Australia, 3: LGMV―Laboratório de Genética Molecular Vegetal, Departamento de Genética, Instituto de Biologia, CCS, Universidade Federal do Rio Janeiro, 21944–970, Ilha do Fundão, Rio de Janeiro, Brasil, Email: margisr@biologia.ufrj.br
English Title: Isolation and RNA silencing of homologues of the RNase L inhibitor in Nicotiana species.
Personal Authors: J
Author Affiliation: Biotechnology Group, Danish Institute of Agricultural Sciences, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark.
Document Title: Plant Science, 2004 (Vol. 167) (No. 6) 1283-1289
Abstract:
Based on the sequences of mammalian RNase L inhibitors (RLI), homologues were identified in Arabidopsis thaliana and Nicotiana plant species. RLI homologue (RLIh) cDNAs were cloned from Nicotiana benthamiana and Nicotiana tabacum and used to induce post-transcriptional gene silencing (PTGS) in Nicotiana species. Downregulation of RLIh in N. benthamiana plants by virus-induced gene silencing had severe effects on plant morphology and plant growth but did not affect the susceptibility of the plant to infection by Potato virus Y (PVY). Upon transformation of N. benthamiana and N. tabacum with an inverted repeat of RLIh only one transformant, X#2.1, displayed an abnormal phenotype characterized by reduced growth and distorted leaves with white spots. Northern blotting revealed the presence of RLIh-specific small interfering RNAs in X#2.1 but not in 14 other transformants with a normal phenotype. Based on our observations we propose that RLIh has an essential role in plant growth and development.
Publisher: Elsevier Science Ltd
About CAB Abstracts
CAB Abstracts is a unique and informative resource covering everything from Agriculture to Entomology to Public Health. In April 2006 we published our 5 millionth abstract, making it the largest and most comprehensive abstracts database in its field.
There are numerous records and resources related to this abstract in the CAB Abstracts database. At this time, your institution does not subscribe to CAB Direct so you cannot access them. To find out more about this exciting resource, and how to subscribe, please click here.
CAB Abstracts contains a wealth of information on the following items that are related to the abstract above:
clones, complementary DNA, DNA cloning, enzyme inhibitors, genes, genetic engineering, genetic transformation, growth, leaves, nucleotide sequences, phenotypes, plant development, plant diseases, plant morphology, plant pathogens, ribonucleases, RNA, susceptibility, tobacco, transgenic plants, gene silencing, potato virus Y, Potyviridae, Potyvirus, cDNA, genetic manipulation, phytopathogens, RNASE, ribonucleic acid, genetically engineered plants, genetically modified plants, GMOs, Arabidopsis thaliana, Nicotiana, Nicotiana benthamiana, Nicotiana tabacum, plant viruses, plants, potato Y potyvirus
RNas eLはアラビドプシスのIre1と構造が似ているらしい
http://www.arabidopsis.org/servlets/TairObject?id=33602&type=locus
